Announcements

San Francisco-based startup E2B, with Czech Founders, raises $21M Series A led by Insight Partners to scale AI agent infrastructure

by
Tom Cironis
July 29, 2025
Czech-founded startup E2B has successfully raised $21 (€18.16) million Series A. The round was led by Insight Partners, alongside Decibel Partners, Sunflower Capital, KAYA VC, and angel investors, including Scott Johnston, former CEO of Docker.

The investment will enable E2B to accelerate the expansion of its cloud infrastructure offerings, enhance its open-source interoperability with other infrastructure providers, and scale its enterprise-focused engineering and go-to-market teams globally.

This funding round reflects E2B’s vision of having dedicated infrastructure for agents with secure and safe sandboxes that can spin up rapidly, providing computer-use features for agents to carry out complex, multi-step tasks. This vision is embraced by 88% of Fortune 100 companies that have already signed up with E2B.

E2B’s customer list already includes leading AI innovators such as Hugging Face, Perplexity, Groq, and Manus. Beyond these, E2B is used by top foundation model providers and Fortune 100 enterprises. These companies leverage E2B’s sandboxed cloud environments to run secure, complex agentic workflows that can reduce deployment times, boost security, and accelerate innovation.

The expectations we have from AI agents in the enterprise are great. However, we expect them to scale and perform on legacy infrastructure not designed for agentic use,” said Vasek Mlejnsky, Co-founder and CEO of E2B. “E2B solves this by equipping AI agents with safe, scalable, highly performant cloud infrastructure as well as tools that help agents scale in production.” E2B plans to strengthen its open-source sandbox protocol as a universal standard and further develop enterprise-grade modules like secrets vault and monitoring tools.

“Insight Partners is excited to back E2B’s visionary team as they pioneer essential infrastructure for AI agents,” said Praveen Akkiraju, Managing Director at Insight Partners. “Such rapid growth and enterprise adoption can be difficult to achieve, and we believe that E2B’s open-source sandbox standard will become a cornerstone of secure and scalable AI adoption across the Fortune 100 and beyond.”

Insight Partners is a global venture capital and private equity firm headquartered in New York City, with additional offices in London, Tel Aviv, and Palo Alto. It specializes in investing in high-growth technology, software, and internet companies.

The future of enterprise productivity is agent-driven,” added Mlejnsky. “Our goal is to provide the standard runtime infrastructure for this new era of work.”

Subscribe to our newsletter

Join our newsletter to get the best hottest startup tapas.

Popular posts

London Quantum Group launches from stealth with $1.2M to solve one of healthcare AI’s biggest challenges

August 6, 2026

Kristýna Truksová: “The office has to earn the commute”

September 1, 2026

Estonian startup Wayren raises €7.9 million to develop an innovative communications system for the soldier of the future

April 29, 2025

Related posts

Funding will accelerate product development and global deployment of the Wonderful AI OS, as enterprises move from isolated AI use cases to organization-wide transformation.
by
Jakob Ulrych
Rightcharge has secured growth funding to expand across Europe and develop its platform, which simplifies home and public EV charging payments for fleet operators.
by
Jakob Ulrych
Prague-based AI initiative prg.ai is getting a new leader. Luděk Šafář, a technology executive with more than 25 years of experience across global companies including Dell Technologies, Microsoft, Novell, SUSE and SAS, will become the organisation’s new director in October.
by
Jakob Ulrych
For early-stage startups in Eastern Europe, building an international network of investors, mentors, partners and customers often takes time, travel and relationships built across multiple markets. Spotlight 2026 brings that access to Bucharest through a three-day startup program and competition taking place at How to Web Conference, October 6–8.
by
Collaborative Post